Welcome to the lab, and the world of Drosophila. Although you might not realize it, a huge chunk of the knowledge gained in genetics, development, and molecular physiology was obtained through the use of these little flies. Prepare to be astonished at what one can do with this amazing 'designer organism'.

These web pages are designed to be an introduction to what we do, how we do it, and a continuing resource for use while we work on doing it. Basically, we genetically alter flies and then look to see what happens to their synapses. To help us, you will need to know: 1) how to culture and genetically manipulate Drosophila, 2) how to study synapses in flies that don't live because their synapses don't work (e.g. patch-clamp electrophysiology and/or microscopy in late-stage fly embryos).

There are several handy sources for this information:

1) Lab members and other colleagues

2) The 'wee library'

3) Various web pages, such as:

A quickie intro to Drosophila

Maintenance of a Drosophila Lab by Ashburner

Drosophila Genetics primer

Bloomington Stock Center's 'Working with flies'

Read a Classic: 'Drosophila Guide' by Demerec & Kaufmann

A compendium of more fly sites

P-elements in Drosophila

Confocal and laser-scanning microscope info

Olympus Online Microscopy Resource Center (our confocal is an FV500)

The Axon Guide - still the best intro to practical electrophysiology, although getting dated